Technical Report

In Hospitals, Probiotic Cleaning vs. Chemical Cleaners to Reduce Pathogen Resistance

The results showed that probiotic cleaning significantly reduced pathogenic microbial load (by over 80%) and maintained stable effects over time. Probiotic-based cleaners effectively reduced the presence of pathogens on contaminated surfaces, not only inhibiting the growth of pathogenic bacteria and reducing hospital-acquired infections but also avoiding the induction of multidrug resistance. They are environmentally friendly and safe for patients, staff, and cleaning personnel.

Results in a Reduction of Dust Mites by More Than 89% in Fabrics

There are many types of house dust mites in Taiwan, with the most common being the European house dust mite (*Dermatophagoides pteronyssinus*) and the American house dust mite (*Dermatophagoides farinae*). These two species have different habits, leading to variations in their distribution. In northern Taiwan, the European house dust mite predominates and prefers a temperature of 25°C. In central and southern Taiwan, the American house dust mite is more common; it is more heat-tolerant and prefers a temperature of 28°C. Experimental results show that after 14 days of using probiotic products on fabrics, dust mite allergens (not live mites) on the fabrics were significantly reduced compared to untreated fabrics, with a reduction of 89.3% for European house dust mites and 93.7% for American house dust mites.

Using Probiotic Spray Continuously for 7 Days Significantly Reduces Pet Hair Allergens

According to test results, the probiotic-treated cat hair samples showed a 92.8% reduction in the allergen Fel d 1 after 14 days compared to untreated cat hair (left image). For dog hair, probiotic-treated samples showed a 66.7% reduction in the allergen Can f 1 after 14 days compared to untreated dog hair (right image). Probiotics have demonstrated effectiveness in reducing pet hair allergens, offering pet owners a healthier and more comfortable environment, especially for those affected by allergens long-term.

Enhance Sleep Quality for Better Health

Probiotic cleaning products were applied in hotel environments, specifically on beds and mattresses, with cleaning and observations conducted over a month. In treated rooms, a significant reduction in coliform bacteria (an indicator of hygiene and fecal contamination) was observed, with an average decrease of around 50% compared to untreated rooms. *Staphylococcus aureus* levels were reduced by an average of 84% compared to untreated rooms. Daily cleaning with probiotics can create a safer, more hygienic sleeping environment.

This image demonstrates the wound healing mechanism of the probiotics used in NUEXTROPY.

– **Impermeable to bacteria**: The surface layer of the biocomposite material prevents pathogenic bacteria from penetrating, providing a protective barrier.

– **Gas exchange**: The covering layer allows gases (such as oxygen) to pass through, maintaining proper ventilation.

– **Maintains appropriate humidity in the wound**: This layer keeps the wound moist, which aids in the healing process.

– **Absorbs exudate excess**: The material absorbs excess fluid from the wound, preventing excessive exudate.

– **Provides thermal insulation**: The material offers thermal insulation, helping maintain an optimal temperature to accelerate healing.

The enlarged section on the right shows the bacteria and their metabolites within the biocomposite. These bacteria can produce four types of antimicrobial substances:

– **Antibiotics**: Combat pathogenic bacteria.

– **Bacteriocins**: Act against other harmful bacteria.

– **Surfactants**: Have antioxidant effects, neutralizing free radicals.

– **Protease**: Breaks down proteins, aiding in the removal of damaged tissue.

**Summary**

The biocomposite material not only provides a physical barrier to prevent pathogens from entering the wound but also promotes the healing process. The bacteria within the material release metabolites with antimicrobial and antioxidant effects, protecting against free radical damage and helping to reduce inflammation. These active substances reduce oxidative stress, prevent lipid peroxidation, DNA damage, and enzyme inactivation. The aim of this research is to explore new bioactive substances with antioxidant potential to accelerate and enhance wound healing.